LOINC Code 74281-7: M. tuberculosis stim IFN-g and spot count panel (Bld)

What is this code?

LOINC codes identify tests, measurements, observations, survey items, and clinical questions in a standardized way. It is associated with the component Mycobacterium tuberculosis stimulated gamma interferon & spot count panel. It is commonly used with the system or sample type Bld.

When is it used?

• Used in lab systems, EHRs, and clinical data exchange.
• May identify a test, observation, survey item, or clinical document request rather than a diagnosis.
• Status: ACTIVE
• Order vs observation: Order
• Panel type: Panel

What it does not mean

• The code identifies the observation or test, not the actual result.

Key facts

• Mycobacterium tuberculosis stimulated gamma interferon and spot count panel - Blood
• Mycobacterium tuberculosis stimulated gamma interferon & spot count panel
• Interferon gamma release assays (IGRAs) are used for the detection of M. tuberculosis. This panel was created for, but not limited in use to, Oxford Immunotec's T-SPOT.TB assay, which uses enzyme-linked immunospot (ELISPOT) methodology to enumerate M. tuberculosis-sensitized T cells by capturing interferon-gamma (IFN-y) in the vicinity of T cells in the patient's whole blood. Cells are spun and, using the ficoll centrifugation procedure, the polys and RBCs layer together in the bottom fraction with peripheral blood mononuclear cells (PBMCs) in the middle. The PBMCs are pipetted out to yield about 250,000 PBMCs per aliquot. They are incubated with M. tuberculosis specific antigens (ESAT-6, CFP10) in two separate wells, a phytohemagglutinin (PHA) control (a mitogenic stimulator) in another well, and a negative control in a fourth well. Incubation with the antigens allows for stimulation of any sensitized T cells present. Evaluating the number of antigen incubated spots compared to the positive and negative control spots provides a measurement of the abundance of M. tuberculosis sensitive T cells in the patient's blood.
• Acid Fast; Acid Fast Bacilli; Acid fast bacillus; AFB; Blood; ELISPOT; ID; IFN; IGRA; Infectious Disease; InfectiousDisease; M tb; M TB IFN-g; M TB IFN-g+spot Pnl; M tuberculosis; Microbiology; Mtb; Myco; Mycobact; Mycobacterium tuberculosum; Pan; PANEL.MICROBIOLOGY; Panl; Pnl; Point in time; Random; TB; T-SPOT; T-SPOT.TB; Tuberculosis; WB; Whole blood

Where you may see this code

You may see this code in lab systems, lab reports, EHR exports, interoperability feeds, or other structured clinical data exchanges.

Common synonyms

Frequently asked questions